Measurement of Circulating 25-Hydroxy Vitamin D Using Three Commercial Enzyme-Linked Immunosorbent Assay Kits with Comparison to Liquid Chromatography: Tandem Mass Spectrometry Method

Measurement of circulating 25-hydroxy vitamin D using three commercial enzyme linked immunosorbent assay kits with comparison to liquid chromatography - tandem mass spectrometry

First 25-hydroxyvitamin D assay for general chemistry analyzers.

25-Hydroxyvitamin D [25(OH)D], the predominant circulating form of vitamin D, is an accurate indicator of the general vitamin D status of an individual. Because vitamin D deficiencies have been linked to several pathologies (including osteoporosis and rickets), accurate monitoring of 25(OH)D levels is becoming increasingly important in clinical settings. Current 25(OH)D assays are either chroma...

Method evaluation study of a new generation of vitamin D assays

INTRODUCTION Recently several diagnostic manufacturers have launched new 25-hydroxy-vitamin D (25[OH]D) assays, which are aligned to the National Institute of Standards and Technology (NIST) Standard Reference Materials (SRM) (NIST, Gaithersburg, Maryland). The aim of this study was to compare the performance of one liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, one enzyme li...

Deoxynivalenol and its conjugates in beer: a critical assessment of data obtained by enzyme-linked immunosorbent assay and liquid chromatography coupled to tandem mass spectrometry.

Enzyme-linked immunosorbent assays (ELISAs) are often employed for the control of deoxynivalenol (DON) in barley and other intermediates involved in beer production chain. Because of the occurrence of high levels of DON-3-glucoside (DON-3-Glc) in malt and beer that have been reported for the first time in our earlier study, research focused on the accuracy of DON determination by immunoassays i...

Correlation study of enzyme-linked immunosorbent assay and high-performance liquid chromatography/tandem mass spectrometry for the determination of N-methylcarbamate insecticides in baby food

In this work, a correlation study of monoclonal antibody-based enzyme-linked immunosorbent assays (ELISAs) and a liquid chromatography–electrospray mass spectrometric (HPLC/ESI/MS/MS) method for the determination of N-methylcarbamate insecticides carbofuran, carbaryl and methiocarb in fruit baby food is presented. The comparison of performance characteristics of the two methods was carried out ...